Journal:

Article Title: Mutations in APC, Kirsten-ras, and p53--alternative genetic pathways to colorectal cancer

doi: 10.1073/pnas.122612899

Figure Lengend Snippet: A model of the genetic changes required for progression from adenoma to carcinoma in the development of colorectal cancer (6). The proposed order of mutations in APC, K-ras, p53, and the DNA MMR genes is illustrated.

Article Snippet: Genomic DNA for mutation analysis was extracted from each tumor tissue with the Wizard Genomic DNA Purification kit (Promega) according to the manufacturer's instructions.

Techniques:

Journal: Journal of Personalized Medicine

Article Title: Drug Resistance and Molecular Characteristics of Mycobacterium tuberculosis : A Single Center Experience

doi: 10.3390/jpm12122088

Figure Lengend Snippet: Sequence of detection site on membrane strip. MTC, Mycobacterium tuberculosis complex; CC, quality control site; the rest were non-tuberculous mycobacterium detection sites. The Chinese in the upper right corner means that you can write numbers in this area to number the membrane strips.

Article Snippet: The process is the same with Mycobacterium species identification, except the PCR tube and membrane strip ( Mycobacterium tuberculosis rifampicin resistance mutation gene detection Kit, Yaneng Biotechnology Co., Ltd, Shenzhen, China).

Techniques: Sequencing, Membrane, Stripping Membranes, Control

Journal: Frontiers in Molecular Biosciences

Article Title: Sequence- and structure-specific RNA oligonucleotide binding attenuates heterogeneous nuclear ribonucleoprotein A1 dysfunction

doi: 10.3389/fmolb.2023.1178439

Figure Lengend Snippet:

Article Snippet: Additionally, for this study we mutated amino acid R55A in OptoA1 using HiFi DNA assembly cloning (NEBuilder HiFi DNA Assembly Cloning Kit, New England BioLabs) to generate N-pmCry2PHR-A1 (R55A)-mCherry-C.

Techniques: Emulsion, Lysis, Protease Inhibitor, Protein Extraction, Modification, Membrane, Staining, Western Blot, Clone Assay, Recombinant, Software, Microscopy